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Team:Edinburgh/Sequences
From 2011.igem.org
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In any case this is good practice for gene annotation and thinking about reverse complimentary sequences and such. | In any case this is good practice for gene annotation and thinking about reverse complimentary sequences and such. | ||
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==Biobrick prefix, suffix, scar== | ==Biobrick prefix, suffix, scar== | ||
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* Sequence based on our actual sequencing data... but some noisy data has been ignored and sequence based on the above. | * Sequence based on our actual sequencing data... but some noisy data has been ignored and sequence based on the above. | ||
* Sequence includes BioBrick prefix and suffix (blue) as well as the BglII site (bold), which is partly in the prefix | * Sequence includes BioBrick prefix and suffix (blue) as well as the BglII site (bold), which is partly in the prefix | ||
| - | * Both forward and reverse sequencing reactions failed to give good info about the suffix; | + | * Both forward and reverse sequencing reactions failed to give good info about the suffix; this seems a little suspicious to me [AC], but I have simply added the standard suffix. |
Revision as of 17:23, 10 June 2011
It may or may not be useful to have annotated nucleotide sequences of various stuff that we use. Some of this information can be retrieved from the Registry, but it may be useful to have it in a more nicely usable form. Copying DNA sequences from the Registry using the web-browser's normal copy function is strangely broken...
In any case this is good practice for gene annotation and thinking about reverse complimentary sequences and such.
Contents |
Biobrick prefix, suffix, scar
- gaattcgcggccgcttctagag - prefix, long
- gaattcgcggccgcttctag - prefix, short
- tactagtagcggccgctgcag - suffix
- tactag - scar, short
- tactagag - scar, long
Restriction sites
- gaattc - EcoRI (prefix 1)
- tctaga - XbaI (prefix 2)
- actagt - SpeI (suffix 1)
- ctgcag - PstI (suffix 2)
- gcggccgc - NotI (in both prefix and suffix)
- agatct - BglII (not part of RFC10, we use it internally after the prefix)
pSB1C3 vector
- This is the official sequence from the Registry ([http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 here])
- Red is CmlR coding sequence ([http://en.wikipedia.org/wiki/Chloramphenicol_acetyltransferase chloramphenicol acetyltransferase])
- Green is the the verification primer binding sites
- Blue is restriction sites for the 4 main enzymes (EcoRI, XbaI, SpeI, PstI)
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct
tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata
acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga
gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct
gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt
aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag
acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac
ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg
ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca
gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc
taaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat
ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaatt
cattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc
catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc
tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac
tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg
aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata
tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatc
cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagtt
ggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca
gaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag
Plac LacZ insert (clone 2)
- A modified version (seems to lack about 45 bases at the start) of [http://partsregistry.org/wiki/index.php?title=Part:BBa_J33207 BBa_J33207]
- Sequence based on our actual sequencing data... but some noisy data has been ignored and sequence based on the above.
- Sequence includes BioBrick prefix and suffix (blue) as well as the BglII site (bold), which is partly in the prefix
- Both forward and reverse sequencing reactions failed to give good info about the suffix; this seems a little suspicious to me [AC], but I have simply added the standard suffix.
gaattcgcggccgcttctagagatctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
gttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactggga
aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacag
ttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagtagcggccgctgcag
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