Team:Cornell/Week 1

(Difference between revisions)

Revision as of 01:32, 16 September 2011

Results | Protocol | Notebook | Parts Submitted

June 5th - June 11th

Cornell iGEM Bootcamp Training Session (Day 1)

Part I

1. PCR setup

2. Run an agarose gel and gel purify the samples using Qiagen Kit.

3. Quantify the samples using NanoDrop.

4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.

5. PCR clean up the samples and quantify using NanoDrop.

Part II

1. Miniprep the overnight culture using Qiagen Kit.

2. Quantify the samples using NanoDrop.

3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.

4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.

5. Run the samples on the agarose gel.

6. Cut out the band of right size, gel purify, and quantify with NanoDrop.

Weill Hall Lab Space Introduction by Dr. Archer

Cornell iGEM Bootcamp Training Session (Day 2)

1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.

2. Desalt the ligation samples on a membrane.

3. Electroporate the samples in the electrocompetent cells.

4. Shake the cells in 37°C shaker for 1 hour.

5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.

CU GEM Bootcamp Training Session (Day 3)

1. Check the colonies on the plates

Team Meeting

@@ Line 30: / Line 30: @@
 :'''Weill Hall Lab Space Introduction by Dr. Archer'''
-:'''CU GEM Bootcamp Training Session (Day 2)'''
+:'''Cornell iGEM Bootcamp Training Session (Day 2)'''
 :::1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.
 :::2. Desalt the ligation samples on a membrane.