Week 13

--Monday 9/05/11--

After a weekend of more runs, we got together and have decided to reevaluate our methods for collecting data. After looking at the results from the 2%, and seeing that it wasn't too different from the 1% run, we've decided to keep our arabinose testing range between 0% and 1%. As for IPTG levels, however, we're going to start trying something different. So far, we've been inducing at 0 mM, 0.25 mM, 0.5 mM, and 1 mM. We are instead going to try 0 mM, 1 mM, 2 mM, and 5 mM IPTG. This will allow us to see if the current levels of IPTG are sufficient for inducing the expression GFP through derepressing the repressor, or if we need to do more runs with these higher concentrations of IPTG.