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Sensor: Week 3 May 30-June 4
From 2011.igem.org
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(→Testing cI Repressor, Day 6) |
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|B0030<br />B0032<br />C0051 from kit | |B0030<br />B0032<br />C0051 from kit | ||
|- | |- | ||
| - | |Colony Growth | + | |Colony Growth (for sequencing) |
|B0031+C0051<br />B0033+C0051 | |B0031+C0051<br />B0033+C0051 | ||
|} | |} | ||
| + | |||
| + | ==Tuesday== | ||
| + | ===Testing cI Repressor, Day 7=== | ||
| + | The sensor group performed miniprep from the cultures created 5/30/2011. Two RBS and cI repressor constructs were sent to sequencing while assembly was repeated for the other two RBS and the cI repressor since this assembly from last week failed. | ||
| + | {|border="1" | ||
| + | !Assembly Step | ||
| + | |colspan="2" align="center"|'''Parts Involved with Assembly Step''' | ||
| + | |- | ||
| + | |rowspan="2"|Miniprep | ||
| + | |For Sequencing: | ||
| + | |B0031+C0051<br />B0033+C0051 | ||
| + | |- | ||
| + | |For Assembly: | ||
| + | |C0051<br />B0030<br />B0032 | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Insert using XbaI and PstI: | ||
| + | |C0051 | ||
| + | |- | ||
| + | |Vector using SpeI and PstI | ||
| + | |B0030<br />B0032 | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|B0030+C0051<br />B0032+C0051 | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2"|The above ligations were transformed into<br />Escherichia coli cells and plated onto<br />Amp resistant plates | ||
| + | |} | ||
| + | |||
| + | |||
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 15:23, 10 June 2011
Contents |
Monday
Testing cI Repressor, Day 6
The sensor group ran PCR, transformed, and cultured ribosome binding sites from the registry as well as the cI repressor. The sensor group also prepared for miniprep and sequencing for tomorrow.
| Assembly step | Parts Ivolved with Assembly Step |
|---|---|
| PCR | C0051 from kit |
| Transform/Culture | B0030 B0032 C0051 from kit |
| Colony Growth (for sequencing) | B0031+C0051 B0033+C0051 |
Tuesday
Testing cI Repressor, Day 7
The sensor group performed miniprep from the cultures created 5/30/2011. Two RBS and cI repressor constructs were sent to sequencing while assembly was repeated for the other two RBS and the cI repressor since this assembly from last week failed.
| Assembly Step | Parts Involved with Assembly Step | |
|---|---|---|
| Miniprep | For Sequencing: | B0031+C0051 B0033+C0051 |
| For Assembly: | C0051 B0030 B0032 | |
| Restriction Digest | Insert using XbaI and PstI: | C0051 |
| Vector using SpeI and PstI | B0030 B0032 | |
| Ligation | B0030+C0051 B0032+C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates | |
