Team:Lethbridge/Notebook/Lab Work/Group1
From 2011.igem.org
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=April= | =April= | ||
==April 1, 2011== | ==April 1, 2011== | ||
- | < | + | <i> Confirmation Gel of I1343, B0034, C0040 & B0015</i> |
- | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4. | + | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4. |
- | <br>- A gel was made following the 1% Agarose Gel Protocol (100mL). | + | <br>- A gel was made following the 1% Agarose Gel Protocol (100mL). |
- | <br>- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder. | + | <br>- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder. |
- | <br>- The gel ran at 120V for 60 minutes. | + | <br>- The gel ran at 120V for 60 minutes. |
- | <br>- The gel was then stained in Ethidium Bromide for 10 minutes. | + | <br>- The gel was then stained in Ethidium Bromide for 10 minutes. |
- | <br>- Results: The parts were confirmed with the correct sizes. | + | <br>- Results: The parts were confirmed with the correct sizes. |
==April 2, 2011== | ==April 2, 2011== | ||
- | < | + | <i>Assembly of I1343 & B0034 and C0040 & B0015</i> |
- | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream. | + | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream. |
- | <br>- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1. | + | <br>- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1. |
- | <br>- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1. | + | <br>- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1. |
- | <br>- Both ligations were transformed following the Transformation Protocol 1. | + | <br>- Both ligations were transformed following the Transformation Protocol 1. |
- | + | ||
==April 21, 2011== | ==April 21, 2011== | ||
==April 23, 2011== | ==April 23, 2011== | ||
- | ==April 27, 2011 == | + | ==April 27, 2011== |
==April 28, 2011== | ==April 28, 2011== |
Revision as of 19:03, 15 September 2011
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