Team:EPF-Lausanne/Our Project/T7 promoter variants

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To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence.  
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In order to add an extra level of control over the expression of our reporter (Lysis or RFP), we proceeded to manufacture a family of new T7 promoter variants exhibit a wide range of strengths and efficiencies compared to the wildtype.  
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We began with the consensus T7 promoter sequence and made
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A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter.
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Revision as of 18:43, 15 September 2011

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