Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization

From 2011.igem.org

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Revision as of 15:28, 15 September 2011



The system is customizable!!
We know how important flexibility within molecular biology is, however setting up a standard often entails rigidity.
We therefore here provide a guide on how to customize the Plug’n’Play assembly standard.


When do you need to customize the system?

• When a desired biological part is not yet a part of the Plug’n’Play with DNA kit
• When scar-free assembly is required (e.g. protein fusion)
• When you want to introduce mutations




Creating new parts




 



Seamless assembly


 

Site-directed mutagenesis












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