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Team:EPF-Lausanne/Our Project/T7 promoter variants
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To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence. | To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence. | ||
| - | + | A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter. | |
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Revision as of 12:07, 15 September 2011
T7 promoter variants
To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence.
A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter.
