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Team:KULeuven/Death
From 2011.igem.org
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<h3>Modeling Celldeath</h3> | <h3>Modeling Celldeath</h3> | ||
| - | <br><h2>1. | + | <br><h2>1. Description of the cell death system</h2> |
| - | + | ||
| - | For a full description, we refer to the extended project page. This section shows only a brief summary of the cell death description. | + | <br><p>For a full description, we refer to the extended project page. This section shows only a brief summary of the cell death description. |
| - | The cell death mechanism we use, will be induced when stimulus 1 (L-arabinose) or stimulus 2 (lactose) and at the same time a cold temperature is applied. | + | <br><p>The cell death mechanism we use, will be induced when stimulus 1 (L-arabinose) or stimulus 2 (lactose) and at the same time a cold temperature is applied. |
| - | Procedure: We grow the bacteria at the optimal temperature of 37 degrees. Then we produce the protein we wish by applying the correct stimulus, e.g., L-arabinose to produce AFP. After synthesizing the desired amount of protein, we lower the temperature to induce the cell death mechanism. | + | <br><p>Procedure: We grow the bacteria at the optimal temperature of 37 degrees. Then we produce the protein we wish by applying the correct stimulus, e.g., L-arabinose to produce AFP. After synthesizing the desired amount of protein, we lower the temperature to induce the cell death mechanism. |
<br><p>CELL DEATH SCHEME | <br><p>CELL DEATH SCHEME | ||
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<br><p>BIOBRICKS CELL DEATH | <br><p>BIOBRICKS CELL DEATH | ||
| - | The promoters for production of the ribolock Colicin E2 operon (Bba_K131009) are two hybrid promoters: pLac-LuxR promoter and pLux-CI promoter. We describe the promoter kinetics in this subsystem with simplified mass equations. This is a simplification because hill kinetics will approach better the transcription in vivo than mass equations. In the full model, hill kinetics is applied. | + | <br><p>The promoters for production of the ribolock Colicin E2 operon (Bba_K131009) are two hybrid promoters: pLac-LuxR promoter and pLux-CI promoter. We describe the promoter kinetics in this subsystem with simplified mass equations. This is a simplification because hill kinetics will approach better the transcription in vivo than mass equations. In the full model, hill kinetics is applied. |
<br><p>FIGURE 1: pLac-luxR hybrid promotor | <br><p>FIGURE 1: pLac-luxR hybrid promotor | ||
Revision as of 10:15, 15 September 2011
Modeling Celldeath
1. Description of the cell death system
For a full description, we refer to the extended project page. This section shows only a brief summary of the cell death description.
The cell death mechanism we use, will be induced when stimulus 1 (L-arabinose) or stimulus 2 (lactose) and at the same time a cold temperature is applied.
Procedure: We grow the bacteria at the optimal temperature of 37 degrees. Then we produce the protein we wish by applying the correct stimulus, e.g., L-arabinose to produce AFP. After synthesizing the desired amount of protein, we lower the temperature to induce the cell death mechanism.
CELL DEATH SCHEME
BIOBRICKS CELL DEATH
The promoters for production of the ribolock Colicin E2 operon (Bba_K131009) are two hybrid promoters: pLac-LuxR promoter and pLux-CI promoter. We describe the promoter kinetics in this subsystem with simplified mass equations. This is a simplification because hill kinetics will approach better the transcription in vivo than mass equations. In the full model, hill kinetics is applied.
FIGURE 1: pLac-luxR hybrid promotor
FIGURE 2: plux-cI promotor
CelldeathODE.pdf
PARAMETER TABLE
