Transformed lumazine synthase - dt in pSB1C3 into DH5∝
Transformed pBAD-RBS in pSB1C3 into DH5∝
June 24th, 2011
Picked cells from June 21st transformation
June 25th, 2011
Minipreped Cells picked on June 25th, 2011
June 28th
Restriction digest and ran on gel
Lumazine - dt construct successfully inserted into pSB1C3
Glycerol stocked.
Add Gel Picture
July 8th
Performed antibiotic assay on lumazine - dt in pSB1C3
Samples from one colony grew in Kanamicyn; this stock was discarded
PCR
PCR'd out lumazine - dt
Used Prefix and antisense suffix
Ran samples on Gel to confirm sizes
July 10th, 2011
PCR'd out Lumazine Synthase - Dt and RFP
Ran on a 1% agarose gel for 45min at 70volts
July 25th, 2011
ran 4 samples on an agarose gel
Preformed a restriction with EcoRI and PstI for Ryan
July 28th, 2011
picked cells from plates and ran a Colony PCR of:
k331033-k331035 in pSB1C3
P0440-K331033 in pSB1A2
J04500-LUMAZINE SYNTHASE-DT in pSB1AK3
pBAD-P0440 in pSB1A2
LUMAZINE SYNTASE-DT-pBAD in pSB1A2
July 29th, 2011
Ran PCR samples from July 28th on a 1X TBE Agarose gel
For control comparision of parts from July 28th PCR we ran a PCR of:
K331035
K331033
Lumazine synthase - dt
P0440
Control
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