Team:Lethbridge/Notebook/Lab Work/Group3

From 2011.igem.org

(Difference between revisions)
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==July 8th==
==July 8th==
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Performed antibiotic assay on lumazine - dt in pSB1C3  
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Performed antibiotic assay on lumazine - dt in pSB1C3 <br />
Samples from one colony grew in Kanamicyn; this stock was discarded
Samples from one colony grew in Kanamicyn; this stock was discarded
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[[Team:Lethbridge/Notebook|PCR]]
+
[[Team:Lethbridge/Notebook|PCR]] <br />
-
PCR'd out lumazine - dt
+
PCR'd out lumazine - dt <br />
Used Prefix and antisense suffix
Used Prefix and antisense suffix

Revision as of 02:10, 15 September 2011



Contents

June

June 21st, 2011

Restriction, Ligation, and Transformation Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

1. Restriction

  • Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
  • Restricted destination plasmid pSB1C3 with EcoR1 and Pst1

2. Ligation

  • Ligated lumazine synthase-dt into destination plasmid pSB1C3
  • Ligated pBAD-RBS into destination plasmid pSB1C3

3. Transformation

  • Transformed lumazine synthase - dt in pSB1C3 into DH5∝
  • Transformed pBAD-RBS in pSB1C3 into DH5∝

June 24th, 2011

Picked cells from June 21st transformation

June 25th, 2011

Minipreped Cells picked on June 25th, 2011

June 28th

Restriction digest and ran on gel
Lumazine - dt construct successfully inserted into pSB1C3 Glycerol stocked.

Add Gel Picture

July 8th

Performed antibiotic assay on lumazine - dt in pSB1C3
Samples from one colony grew in Kanamicyn; this stock was discarded

PCR
PCR'd out lumazine - dt

Used Prefix and antisense suffix
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