Revision as of 02:03, 15 September 2011

June

Restriction, Ligation, and Transformation Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

- Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
- Restricted destination plasmid pSB1C3 with EcoR1 and Pst1

- Ligated lumazine synthase-dt into destination plasmid pSB1C3
- Ligated pBAD-RBS into destination plasmid pSB1C3

- Transformed lumazine synthase - dt in pSB1C3 into DH5∝
- Transformed pBAD-RBS in pSB1C3 into DH5∝

Picked cells from June 21st transformation

Minipreped Cells picked on June 25th, 2011

Restriction digest and ran on gel Lumazine - dt construct successfully inserted into pSB1C3 Glycerol stocked.

Add Gel Picture

Performed antibiotic assay on lumazine - dt in pSB1C3 Samples from one colony grew in Kanamicyn; this stock was discarded

PCR: PCR'd out RFP and lumazine

@@ Line 43: / Line 43: @@
 Lumazine synthase - double terminator and pBAD - RBS into pSB1C3
-# [[Team:Lethbridge/Notebook|Restriction]]
+. [[Team:Lethbridge/Notebook|Restriction]]
 #* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
 #* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
-## [[Team: Lethbridge/Notebook|Ligation]]
+. [[Team: Lethbridge/Notebook|Ligation]]
 #* Ligated lumazine synthase-dt into destination plasmid pSB1C3
 #* Ligated pBAD-RBS into destination plasmid pSB1C3
-### [Team:Lethbridge/Notebook|Transformation]]
+. [[Team:Lethbridge/Notebook|Transformation]]
 #* Transformed lumazine synthase - dt in pSB1C3 into DH5&prop;
 #* Transformed pBAD-RBS in pSB1C3 into DH5&prop;