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Team:Lethbridge/Notebook/Lab Work/Group3
From 2011.igem.org
(Difference between revisions)
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| - | =June= | + | =June= |
| - | ==June 21st, 2011== | + | ==June 21st, 2011== |
| - | Restriction, Ligation, and Transformation | + | Restriction, Ligation, and Transformation |
| - | Lumazine synthase - double terminator and pBAD - RBS into pSB1C3 | + | Lumazine synthase - double terminator and pBAD - RBS into pSB1C3 |
# [[Team:Lethbridge/Notebook|Restriction]] | # [[Team:Lethbridge/Notebook|Restriction]] | ||
| - | #* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1 | + | #* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1 |
| - | #* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 | + | #* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 |
| - | ## [[Team: Lethbridge/Notebook|Ligation]] | + | ## [[Team: Lethbridge/Notebook|Ligation]] |
#* Ligated lumazine synthase-dt into destination plasmid pSB1C3 | #* Ligated lumazine synthase-dt into destination plasmid pSB1C3 | ||
#* Ligated pBAD-RBS into destination plasmid pSB1C3 | #* Ligated pBAD-RBS into destination plasmid pSB1C3 | ||
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==June 24th, 2011== | ==June 24th, 2011== | ||
| - | Picked cells from June 21st transformation | + | Picked cells from June 21st transformation |
==June 25th, 2011== | ==June 25th, 2011== | ||
| - | Minipreped Cells picked on June 25th, 2011 | + | Minipreped Cells picked on June 25th, 2011 |
==June 28th== | ==June 28th== | ||
Restriction digest and ran on gel | Restriction digest and ran on gel | ||
| - | Lumazine - dt construct successfully inserted into pSB1C3 | + | Lumazine - dt construct successfully inserted into pSB1C3 |
Glycerol stocked. | Glycerol stocked. | ||
=ADD GEL PICTURE= | =ADD GEL PICTURE= | ||
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==July 8th== | ==July 8th== | ||
| - | Performed antibiotic assay on lumazine - dt in pSB1C3 | + | Performed antibiotic assay on lumazine - dt in pSB1C3 |
Samples from one colony grew in Kanamicyn; this stock was discarded | Samples from one colony grew in Kanamicyn; this stock was discarded | ||
PCR: PCR'd out RFP and lumazine | PCR: PCR'd out RFP and lumazine | ||
Revision as of 02:02, 15 September 2011
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