Team:Lethbridge/Notebook/Lab Work/Group3

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#* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 <br />
#* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 <br />
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# [[Team: Lethbridge/Notebook|Ligation]]
+
## [[Team: Lethbridge/Notebook|Ligation]]
#* Ligated lumazine synthase-dt into destination plasmid pSB1C3
#* Ligated lumazine synthase-dt into destination plasmid pSB1C3
#* Ligated pBAD-RBS into destination plasmid pSB1C3
#* Ligated pBAD-RBS into destination plasmid pSB1C3
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 +
### [Team:Lethbridge/Notebook|Transformation]]
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#* Transformed lumazine synthase - dt in pSB1C3 into DH5&prop;

Revision as of 01:47, 15 September 2011



=June=
==June 21st==

Restriction, Ligation, and Transformation
Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

  1. Restriction
    • Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
    • Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
    1. Ligation
    • Ligated lumazine synthase-dt into destination plasmid pSB1C3
    • Ligated pBAD-RBS into destination plasmid pSB1C3
      1. [Team:Lethbridge/Notebook|Transformation]]
    • Transformed lumazine synthase - dt in pSB1C3 into DH5∝
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