Team:Lethbridge/Notebook/Lab Work/Group3

From 2011.igem.org

(Difference between revisions)
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Restriction, Ligation, and Transformation <br />
Restriction, Ligation, and Transformation <br />
-
Lumazine synthase - double terminator - pBAD - RBS into pSB1C3 <br />
+
Lumazine synthase - double terminator and pBAD - RBS into pSB1C3 <br />
# [[Team:Lethbridge/Notebook|Restriction]]   
# [[Team:Lethbridge/Notebook|Restriction]]   
-
#* Restricted lumazine synthase - dt - pBAD - RBS out of pSB1T3 with EcoR1 and Pst1 <br />
+
#* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1 <br />
#* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 <br />
#* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1 <br />
 +
 +
## [[Team: Lethbridge/Notebook|Ligation]]
 +
#* Ligated lumazine synthase-dt into destination plasmid pSB1C3
 +
#* Ligated pBAD-RBS into destination plasmid pSB1C3

Revision as of 01:42, 15 September 2011



=June=
==June 21st==

Restriction, Ligation, and Transformation
Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

  1. Restriction
    • Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
    • Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
    1. Ligation
    • Ligated lumazine synthase-dt into destination plasmid pSB1C3
    • Ligated pBAD-RBS into destination plasmid pSB1C3
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