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Team:Lyon-INSA-ENS/Realisation/Protocols
From 2011.igem.org
(Difference between revisions)
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/f/fd/CaCl2_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
<br/> <br/> | <br/> <br/> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/6/65/Fermentas_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
<br/> <br/> | <br/> <br/> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/9/9d/Ligation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/4/4f/NucleoSpin_Plasmid_QuickPure.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/f/f8/Ozyme_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/7/7f/Pouring_of_plates.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/5/53/QUIGen_midiprep_kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/6/67/QUIGen_Miniprep_Kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/2/2a/Storage_of_strains.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/c/c7/TSS_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
Revision as of 09:51, 14 September 2011
General culture conditions
Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.
The composition of the different media we have used is the following :
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water
