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Team:Washington/Protocols/Glycerol Stocks
From 2011.igem.org
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''For the long-term storage of microbial cultures'' | ''For the long-term storage of microbial cultures'' | ||
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1. Grow overnight culture (1 mL) of your cells in selective media | 1. Grow overnight culture (1 mL) of your cells in selective media | ||
Revision as of 01:36, 14 September 2011
Glycerol Stocks
For the long-term storage of microbial cultures
Make sure that you do not contaminate glycerol stocks
1. Grow overnight culture (1 mL) of your cells in selective media
2. Mix 750 uL of your culture with 750 uL of 40% glycerol. Immediately place at -80 C (glycerol is toxic to cells and prolonged exposure to it kills them at RT).
If you need to take from the stocks, do not thaw - just scrape ice off of the top with a sterile loop and smear across a plate.
