Team:Lyon-INSA-ENS/Realisation/Notebook/Week1

From 2011.igem.org

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<FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgBAEFG</b> </FONT> <br><br>
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Culture of NM 522 cells for later transformations
Culture of NM 522 cells for later transformations
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<FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgBAEFG</b> </FONT> <br><br>
 
<b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.
<b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.

Revision as of 14:54, 13 September 2011







Week 1


From Thursday the 16th of June to Friday the 17th of June 2011





Thursday


Culture of NM 522 cells for later transformations





Friday


Extraction of plasmid with NicoT gene from filter paper. Transformation of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.














ENS assystem Biomérieux INSA INSA