"
Team:Lyon-INSA-ENS/Realisation/Notebook/Week1
From 2011.igem.org
(Difference between revisions)
| Line 45: | Line 45: | ||
<br/> | <br/> | ||
<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| + | |||
| + | <FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgBAEFG</b> </FONT> <br><br> | ||
| + | |||
<b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end. | <b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end. | ||
</p> | </p> | ||
Revision as of 14:52, 13 September 2011
Week 1
From Thursday the 16th of June to Friday the 17th of June 2011
Thursday
Culture of NM 522 cells for later transformations
Friday
PCR and mutagenesis of rcn, csgBA, csgBAEFG
Extraction of plasmid with NicoT gene from filter paper. Transformation of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.
