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Team:Freiburg/Notebook/2 August
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Revision as of 12:45, 13 September 2011
Contact 
Contents |
green light receptor
transformation with quickchanged CcaS
Investigators:Julia
Miniprep
Investigator: Jakob
Miniprep
| Name:
Jakob | Date:
02.08.2011 |
| Continue from Experiment: 27.07.2011
Quickchange CcaR and trafo(28.07.2011) | |
| Project Name:
Green light receptor | |
Documentation:
Why are you doing this experiment? Name the parts which you extract.
| Need the DNA concentration of CcaR a-d |
Describe your results and mistakes and measure the DNA concentration with the Nanodrop and note the results.
|
How did you label your probes and where are they stored?
| In Julias box, CcaR (qc.) a-d |
Testdigest
Investigators: Julia
| Name:
Julia | Date:
02.08.2011 |
| Continue from Experiment 02.08.2011
Miniprep | |
| Project Name: green light receptor, testdigest of CcaR with BamHI | |
For one reaction you need: For Mastermix: Number of samples+2extra
| 4μl | H2O | 20 | |
| 1μl | Buffer, NEB4 | 5 | |
| 1μl | BSA (10x) | 5 | |
| 0,5 μl | Enzym 1 | 1 | BamHI |
| 0,5 μl | Enzym 2 | - | |
| 3 μl | DNA | 3 |
10 μl total volume
Give 3 μl of DNA in an eppi and add 7μl of the mastermix.
Incubate for about 1h at 37°C.
Add 1 μl Loading dye buffer and load the gel.
DNA Gel
Investigator: Jakob
we dont see the expected edges.
- To-do: Repeat testdigest and if necessery quickchange tomorrow.
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
transformation with Ligationreaction(pcyA+terminator) from 1st of august and the pJT122
Investigators:Julia
Precipitator
Oligo order
Investigators: Rüdiger
