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Team:Lyon-INSA-ENS/Realisation/Week2
From 2011.igem.org
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| - | One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.<br/> | + | One transformed colony (NicoT in NM522) seemed correct, the others were probably satellite colonies without the plasmid.<br/> |
Start of 5mL liquid culture of the main colony. <br/> | Start of 5mL liquid culture of the main colony. <br/> | ||
Plating of the satellite colonies to check the presence of the plasmid <br/> | Plating of the satellite colonies to check the presence of the plasmid <br/> | ||
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| - | Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII) | + | Miniprep on the four clones. Verification of plasmid by <b>digestion</b> (EcoRI, HindIII) |
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| - | PCR from genomic DNA from E.coli strain MC4100 to amplify three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2) | + | <b>PCR</b> from genomic DNA from E.coli strain MC4100 to amplify three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2) |
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Revision as of 12:42, 13 September 2011
Week 2
From Monday the 20th of June to Friday the 24th of June 2011
Monday
One transformed colony (NicoT in NM522) seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid
Tuesday
4 more new clones have grown : start of 5mL liquid cultures on these clones.
Wednesday
Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)
Thursday
PCR from genomic DNA from E.coli strain MC4100 to amplify three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)
Friday
