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Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme
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Revision as of 10:10, 13 September 2011
Ozyme digestion
This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.
Procedure
1. In an eppendorf tube, add the following solutions in any order :
-11µL water
-10µL DNA
-2.5µL NE Buffer 2
-0.5µL BSA X50
2. Add 0.5µL of each one of the desired enzymes.
3. Incubate at 37°C for 10mn
4. Inactivate the enzymes by a 20mn incubation at 80°C
