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Team:Lyon-INSA-ENS/Realisation/Protocols
From 2011.igem.org
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| - | <p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br> | + | <p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br><br> |
Ampicilin (Amp) : 100µg/mL <br> | Ampicilin (Amp) : 100µg/mL <br> | ||
Chloramphenicol (Cm) : 20µg/mL <br> | Chloramphenicol (Cm) : 20µg/mL <br> | ||
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The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br> | The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br> | ||
| - | <p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br> | + | <p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br> |
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br> | LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br> | ||
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br> | M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br> | ||
Revision as of 10:07, 13 September 2011
General culture conditions
Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.
The composition of the different media we have used is the following :
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water
