Team:UST-Beijing/Parts
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- | < | + | <td width="785"><img src="https://static.igem.org/mediawiki/igem.org/0/0d/USTBpart.JPG" width="794" height="763"></td> |
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- | + | <td><img src="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" alt="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" width="558" height="212"></td> | |
- | + | <td>PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part, K603000 designed by Danyang Wang, was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</td> | |
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Latest revision as of 02:16, 13 September 2011
PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part, K603000 designed by Danyang Wang, was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens. | |