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Team:Cornell/Week 15
From 2011.igem.org
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==Monday, September 12== | ==Monday, September 12== | ||
Afternoon lab work done by: Nancy Li, Charlie Chung | Afternoon lab work done by: Nancy Li, Charlie Chung | ||
| - | :*Prepared | + | :*Prepared ten 3mL LB cultures (with 3µL carbenicillin) of colonies picked from plates dated Sept 3 |
::- RFP + Avi-Tagged pZE12 in DH5α | ::- RFP + Avi-Tagged pZE12 in DH5α | ||
::- vioA + Avi-Tagged pZE12 in DH5α | ::- vioA + Avi-Tagged pZE12 in DH5α | ||
Revision as of 02:15, 13 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 11th - September 17th
Sunday, September 11
~God bless America -- never forget~
Monday, September 12
Afternoon lab work done by: Nancy Li, Charlie Chung
- Prepared ten 3mL LB cultures (with 3µL carbenicillin) of colonies picked from plates dated Sept 3
- - RFP + Avi-Tagged pZE12 in DH5α
- - vioA + Avi-Tagged pZE12 in DH5α
- - vioB + Avi-Tagged pZE12 in DH5α
- - vioE + Avi-Tagged pZE12 in DH5α
- - RFP + Avi-Tagged pZE12 in MC4100
- - vioA + Avi-Tagged pZE12 in MC4100
- - vioB + Avi-Tagged pZE12 in MC4100
- - vioE + Avi-Tagged pZE12 in MC4100
- - Control tube is just a colony with pZE12 (no insert gene)
- - LB with carbenicillin (no inoculation with bacteria)
- Incubating at 37°C in the shaker at Weill Hall
- Cultures are to be lysed for release of natively biotinylated RFP and vio enzymes in characterizing streptavidin-coated microfluidics chips
