Team:Freiburg/Notebook/12 September

From 2011.igem.org

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*44B-S1b
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===Trafo===
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''''Investigators: Sandra'''
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Trafo of the ligated samples on plates with Cm.
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 13:38, 12 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

Minipreps

Investigators: Sandra

Because of the positive testdigest, a miniprep of the PR+GFP was performed. Samples were send for sequencing.


green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep of PR+lovtap-notgate domain

Investigators: Sandra

Miniprep of:

  • 39A
  • 39B
  • 40A
  • 40B
  • 41A
  • 41B
  • 42A
  • 42B
  • 44A
  • 44B

39=PR1, 40=PR2, 41=PR3, 42=PR4, 44=PR6.

2A-assembly: Digestion

Investigators: Sandra


Digestion of the samples from miniprep (10 samples) and digestion of RFP (S1b, S2b).

PR with Lov-Notgate domain:

  • 39A
  • 39B
  • 40A
  • 40B
  • 41A
  • 41B
  • 42A
  • 42B
  • 44A
  • 44B

digested with NehI and PstI.

RFP:

  • S1b
  • S2b

digested with SpeI and PstI.


2A-assembly: Ligation

Ligation of RFP with 39-44.

  • 39A-S1b
  • 39A-S2b
  • 39B-S1b
  • 39B-S2b
  • 40A-S1b
  • 40A-S2b
  • 40B-S1b
  • 40B-S2b
  • 41A-S1b
  • 41A-S2b
  • 41B-S1b
  • 41B-S2b
  • 42A-S1b
  • 42A-S2b
  • 42B-S1b
  • 42B-S2b
  • 44A-S1b
  • 44A-S2b
  • 44B-S1b
  • 44B-S2b


Trafo

'Investigators: Sandra

Trafo of the ligated samples on plates with Cm.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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