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Sensor: Week 2 May 23-27
From 2011.igem.org
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|colspan="2" | The above ligation was transformed into Escherichia<br />coli cells and plated onto Amp resistant plates. | |colspan="2" | The above ligation was transformed into Escherichia<br />coli cells and plated onto Amp resistant plates. | ||
| + | |- | ||
| + | |PCR | ||
| + | |colspan="2" align="center"| B0030<br />B0031<br />B0032<br />B0033 | ||
|} | |} | ||
Revision as of 22:28, 7 June 2011
Monday
| Assembly Step | Parts Involved in Assembly Step |
|---|---|
| PCR | J23113 J23105 J23118 J23100 |
Tuesday
| Assembly Step | Parts Involved in Assembly Step | |
| PCR Purification | J23113 J23105 J23118 J23100 | |
| Miniprep | B0051 | |
| Restriction Digest | Inserts using EcoRI and SpeI: | J23113 J23105 J23118 J23100 B0034+Mcherry |
| Vectors using EcoRI and XbaI: | B0034+C0051 B0015 | |
| Ligation | J23113 and B0034+C0051 J23105 and B0034+C0051 J23118 and B0034+C0051 J23100 and B0034+C0051 B0034+Mcherry and B0015 | |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
| PCR | B0030 B0031 B0032 B0033 | |
