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Team:WarrenCIndpls IN-HS/Notebook
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Kozak Sequence- directs translation of mRNA for more efficiency and accuracy | Kozak Sequence- directs translation of mRNA for more efficiency and accuracy | ||
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Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors | Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors | ||
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Terminator Sequence- signals the end of transcriptoin to RNA polymerase | Terminator Sequence- signals the end of transcriptoin to RNA polymerase | ||
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Vector- Plasmid | Vector- Plasmid | ||
ORI (Origin of Replication) | ORI (Origin of Replication) | ||
Revision as of 17:16, 7 June 2011
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Contents |
Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors
Terminator Sequence- signals the end of transcriptoin to RNA polymerase
Vector- Plasmid
ORI (Origin of Replication)
Selection Markers
-Ura3 is a selection marker for yeast
-Ampicillin is a selection marker for bacteria
