Team:Wageningen UR/Project/Aftermath
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However, our synthesis company had problems with putting the rest of our constructs together. Apparently one of the synthetic sequences was toxic for the <span style="font-style:italic;">E. coli</span> host vector. When verifying the sequence no faultless sequence could be verified. Huge deletions were found in the sequence, indicating that there was something fundamentally wrong with our sequence. Upon closer inspection we found four small separate repeats in our sequence, however we strongly doubt this is the root of our problems. | However, our synthesis company had problems with putting the rest of our constructs together. Apparently one of the synthetic sequences was toxic for the <span style="font-style:italic;">E. coli</span> host vector. When verifying the sequence no faultless sequence could be verified. Huge deletions were found in the sequence, indicating that there was something fundamentally wrong with our sequence. Upon closer inspection we found four small separate repeats in our sequence, however we strongly doubt this is the root of our problems. | ||
- | Due to all these problems the delivery of our synthetic construct was massively delayed. At the moment of writing several parts arrived by mail, | + | Due to all these problems the delivery of our synthetic construct was massively delayed. At the moment of writing several parts arrived by mail, incomplete and useless. After we noticed this incredible delay the decision was made to wrap up the fungal track 'n trace project. Suzette continued to work on several microscopal aspects of fungal hypha, but basically all of our man and womanpower shifted to the Synchrossilator project where we managed to get beautiful results. |
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Revision as of 16:57, 8 September 2011