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Team:DTU-Denmark-2/Project/PlugnplayAssembly
From 2011.igem.org
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| + | <b></b>Making Molecular Biology Easier<br> | ||
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In 2009 the first team from DTU to participate in iGEM introduced the USER fusion Assembly standard. This year we introduce a more standardized version allowing easier use of the system. | In 2009 the first team from DTU to participate in iGEM introduced the USER fusion Assembly standard. This year we introduce a more standardized version allowing easier use of the system. | ||
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We think that iGEM should be about assembling biobricks fast and combining them in any thinkable way with existing or new parts. Unfortunately classical cloning techniques can cause problems and even PCR can be cumbersome if you have little or no laboratory experience. | We think that iGEM should be about assembling biobricks fast and combining them in any thinkable way with existing or new parts. Unfortunately classical cloning techniques can cause problems and even PCR can be cumbersome if you have little or no laboratory experience. | ||
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| - | We therefore introduce a simple and fast way of building new devices or whatever you want with ready to use PCR products. All you have to do is to select your favorite bricks, mix them with the ready to use destination vector and 70 minutes later you are ready to transform your competent E. coli cells. No need to worry if your destination vector has been fully linearized, no need to perform site-directed mutagenesis to remove illegal restriction sites, just simple, fast, and easy. | + | <br> |
| + | We therefore introduce a simple and fast way of building new devices or whatever you want with ready to use PCR products. All you have to do is to select your favorite bricks, mix them with the ready to use destination vector and 70 minutes later you are ready to transform your competent <i>E. coli</i> cells. No need to worry if your destination vector has been fully linearized, no need to perform site-directed mutagenesis to remove illegal restriction sites, just simple, fast, and easy. | ||
Revision as of 20:17, 7 September 2011
Making Molecular Biology Easier
In 2009 the first team from DTU to participate in iGEM introduced the USER fusion Assembly standard. This year we introduce a more standardized version allowing easier use of the system.
We think that iGEM should be about assembling biobricks fast and combining them in any thinkable way with existing or new parts. Unfortunately classical cloning techniques can cause problems and even PCR can be cumbersome if you have little or no laboratory experience.
We therefore introduce a simple and fast way of building new devices or whatever you want with ready to use PCR products. All you have to do is to select your favorite bricks, mix them with the ready to use destination vector and 70 minutes later you are ready to transform your competent E. coli cells. No need to worry if your destination vector has been fully linearized, no need to perform site-directed mutagenesis to remove illegal restriction sites, just simple, fast, and easy.
