Team:Grenoble

From 2011.igem.org

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<h1>Design and principle</h1>
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<h2><a href="#toggle">Toggle Switch</a></h2>
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<h2><a href="#qs">Quorum Sensing</a></h2>
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<h2 id="toggle">Toggle switch explanation</h2>
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<h3>In a few words</h3>
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<h2 id="Paragraphe1">You are welcome !<span>By JB</span></h2>
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Welcome on version 0.3 of our wiki ! You may still find some blank pages but we are filling it day by day.<br/>
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Our project is based on a toggle switch system. So we have to explain how it works. Basically it corresponds to an irreversible choice between two pathways depending on the environment. In reality, the reversibility is possible but very difficult to obtain because of the strength of the interactions and the stability of the system (defined by hysteresis * insérer un lien ici*).  
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Follow the state of our work in the<a href="/Team:Grenoble/Notebook/July">Notebook</a> or learn everything you always wanted to know about Grenoble and tartiflette <a href="/Team:Grenoble/Grenoble" >here !</a>
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<a href="https://static.igem.org/mediawiki/2011/b/b6/Toggle1.png"><img src="https://static.igem.org/mediawiki/2011/b/b6/Toggle1.png" alt="2 pathways toggle switch" title="Choose one way, no turning back !"></a>  
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<h3>Genetically, what is happening ?</h3>
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<a href="http://syntheticbiology.org/">
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<h2 class="lien"id="Paragraphe2">iGEM and the future of synthetic biology<span>By Geoffrey</span></h2>
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Now booming, synthetic biology is a scientific field that divides the world into 2 parts. On the one hand, people who believe it will  science and our life. On the over hand, people who believe that synthetic biology is dangerous and it simply means playing God.
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Contrary to us, bacteria can’t think or make voluntary choices. So how can they choose a pathway? Through our project, we are creating a genetic system which helps bacteria to take such a decision. For that, we exploit chemical components of the environment which influence the bacterial behaviour. By taking into account two proteins repressing each other’s expression (LacI and XR), we create the fundaments of a toggle system that can switch when using two inducers (IPTG and X) in the medium.
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<a href="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png"><img src="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png" alt="Event tree, bacteria out of the lab" title="Event Tree, bacteria are out of the lab ! What would happen ?"></a>
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Like all new domains of science, many questions may be raised about synthetic biology. Albert Einstein said: “It is strange that science which before seemed to be inoffensive will be turned into a nightmare that frightens everyone”. The goal of iGEM competition is to promote synthetic biology in the scientific community, but also to inform the public about this new science. Synthetic biology is a very controlled science to prevent side-effects and many countries such as France move to a strong legislative supervision in order to respect the ethical issues related to the modification of life.
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According to the <a href="http://www.bulletins-electroniques.com/actualites/66814.htm">newsletter of the French Embassy to the United States</a>, the international scientific community recognizes the dangers of synthetic biology and it must identify them precisely. It has especially given to the competition iGEM an important educational role for the scientific community of tomorrow.
 
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However, synthetic biology is a science with an extraordinary potential. Technological advances it could bring are huge. The fields of application are vast, from agriculture to medicine through the environment and energy, synthetic biology offers new possibilities and new alternatives to current technologies. The iGEM competition directly contributes to advance research in all these fields by enabling student teams to contribute in <a href="http://ung.igem.org/Team_Tracks?year=2011">these categories</a>.
 
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"Open source" policy conveyed by iGEM allows the world to take advantage of advances in this field and thus to improve every year the level of competition and projects. And we can say that the objective is on track, because the number of teams participating in this competition is <a href="http://ung.igem.org/Previous_iGEM_Competitions">constantly increasing</a>.
 
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<h2 id="qs">Quorum Sensing</h2>
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<h2 class="lien"id="Paragraphe2">A great human adventure<span>By Félix</span></h2>
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iGEM competition includes about 160 teams (more or less 2000 students) over the world. There will be a first regional jamboree in different regions such as Europe, Asia, America,… and then a third of us will go to Boston for the last jamboree. This will be the first opportunities for most of students to meet people from so many universities at once ! We ‘ll be hosting at the same places, sharing breakfast and even partying together !... We ‘ll be able to discuss abouts many topics related to our fields, share ideas, and to see how others managed their work by watching their presentations.
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<dt>Ultra violet lamp: </dt>
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There is a risk for the eyes and the skin, but the UV lamp is only used to take a picture of our gel after electrophoresis, so we are never directly exposed because there is a protective cover and we wear a mask that shields from UV.
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<dt>Centrifuge:</dt>
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The centrifuges have to be perfectly balanced. All the centrifuges in our lab have detectors that warn the operator in case of imbalance.
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<dt>Autoclave:</dt>
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The operation of the autoclave require a specific training and has only be performed by trained people.
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iGEM competition is not just about doing scientific work in order to get a medal, but is also about doing all of what an engineer or researcher needs to do ! Each team needs to set up contacts and collaboration with other groups. Sharing bricks or helping others is a criteria for winning a gold medal! We also need to get funds in order to finance our work…. So we  must make a project that fits some challenge of our society ! As every one knows, new technologies rise up new ethical issues. This aspect has to be considered on every single project !
 
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Now that we have found an interesting  project  which seems ethic, and can be useful for the society, we are on the way for 4 month of intense work ! Our group is made of 12 people with very different education , that have to understand and synchronise each other. Since we are spread out in multiple places, we already meet once a week in order to coordinate or work. This need of an efficient communication within the team is an exiting part of the project !
 
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Revision as of 16:43, 7 September 2011

Grenoble 2011, Mercuro-Coli iGEM


Design and principle

Toggle Switch

Quorum Sensing

Toggle switch explanation

In a few words

Our project is based on a toggle switch system. So we have to explain how it works. Basically it corresponds to an irreversible choice between two pathways depending on the environment. In reality, the reversibility is possible but very difficult to obtain because of the strength of the interactions and the stability of the system (defined by hysteresis * insérer un lien ici*).
2 pathways toggle switch

Genetically, what is happening ?

Contrary to us, bacteria can’t think or make voluntary choices. So how can they choose a pathway? Through our project, we are creating a genetic system which helps bacteria to take such a decision. For that, we exploit chemical components of the environment which influence the bacterial behaviour. By taking into account two proteins repressing each other’s expression (LacI and XR), we create the fundaments of a toggle system that can switch when using two inducers (IPTG and X) in the medium. Event tree, bacteria out of the lab

Quorum Sensing

Ultra violet lamp:
There is a risk for the eyes and the skin, but the UV lamp is only used to take a picture of our gel after electrophoresis, so we are never directly exposed because there is a protective cover and we wear a mask that shields from UV.
Centrifuge:
The centrifuges have to be perfectly balanced. All the centrifuges in our lab have detectors that warn the operator in case of imbalance.
Autoclave:
The operation of the autoclave require a specific training and has only be performed by trained people.

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