Team:Freiburg/Notebook/7 September

From 2011.igem.org

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'''Investigators: Sophie''' <br/>
'''Investigators: Sophie''' <br/>
yesterday two tubes with LB were inoculated with cells from our S35 glycerol stock(BBa_K322999). Today minipreps were performed.
yesterday two tubes with LB were inoculated with cells from our S35 glycerol stock(BBa_K322999). Today minipreps were performed.
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===PCR===
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'''Investigators: Sophie''' <br/>
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 13:59, 7 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

Ligation

Investigators: Sandra

Ligation of PR1-PR6 with RFP (S1b and S2a). PR1-PR6 were digested with antarctica before start of ligation. Start of ligation at 12:40. Incubation at room temperature for at least 3 hours.

Trafo

Investigators: Sandra

Trafo of:

  • PR1+GFP
  • PR2+GFP
  • PR3+GFP
  • PR4+GFP
  • PR5+GFP
  • PR6+GFP
  • PR1+50b
  • PR2+50b

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

no colonies on the plates

There were no colonies on the plates with ligated parts of the 2A-assembly.

Miniprep

Investigators: Sophie
yesterday two tubes with LB were inoculated with cells from our S35 glycerol stock(BBa_K322999). Today minipreps were performed.

PCR

Investigators: Sophie

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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