6 (June 6, 2011 GDS)

From 2011.igem.org

(Difference between revisions)
(Created page with "Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by i...")
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Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes, test at 1.0 K. Transfer the supernatant to clean tube and ___ 2 volumes (1mL) of room temp ETOH. Vortex and pellet DNA by centrifugation for 2-5 min. at 10 K. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE with RNAseA. Digest 5-10uL as uaual.
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Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes. Transfer the supernatant to clean tube and add 2 volumes (1mL) of room temp ETOH. Mix and pellet DNA by centrifugation for 2-5 min. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE. Digest 5-10uL as usual.
:PNICE--5/12/11
:PNICE--5/12/11
:PNICE (tKAN)--5/23/11
:PNICE (tKAN)--5/23/11

Revision as of 20:53, 6 September 2011

Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes. Transfer the supernatant to clean tube and add 2 volumes (1mL) of room temp ETOH. Mix and pellet DNA by centrifugation for 2-5 min. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE. Digest 5-10uL as usual.

PNICE--5/12/11
PNICE (tKAN)--5/23/11
J04430--5/23/11
J04430--5/12/11
Trans 003-W1--5/12/11
Trans 003-W1--5/23/11

P.P. G.E. S.D.

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