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From 2011.igem.org

Revision as of 19:18, 5 September 2011 (view source) Sallen (Talk | contribs) ← Older edit		Revision as of 20:41, 6 September 2011 (view source) Pjpatel (Talk | contribs) m (grammar) Newer edit →
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	:pNICE SA-15λ TE		:pNICE SA-15λ TE
	:J024430 GE-15λ TE		:J024430 GE-15λ TE
-	:Trans002-5, AB-Resp 30&lambda	+	:Trans002-5, AB-Resp 30λ
	:Trans003-W1 SD, SA-		:Trans003-W1 SD, SA-

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Revision as of 20:41, 6 September 2011

Parth--made fresh TENS

4.5 TE

250λ 10% SDS

250λ 2M NaOH

Spin 1.5 mL (2 tubes) 30"

Pour off sol'n + resuspend

Add: 300λ TENS mix

150λ NaOAc mix

Spin 2.5'

Sup to new tube

Add 1 mL ETOH - mix well

Spin 3' ________

Wash 70% ETOH

Minipreps (2 each) for:

pNICE SA-15λ TE

J024430 GE-15λ TE

Trans002-5, AB-Resp 30λ

Trans003-W1 SD, SA-