While engineering ''E.D. Frosti'' we added several mechanisms to ensure biosafety. When a stimulus (arabinose or lactose) is combined with a cold temperature, a cell death mechanism will kill off the cells. Hereby the bacteria will be killed but the produced proteins stay active on their cell surface for some period of time. We thought about the dangers that ''E.D. Frosti'' could entail for the environment and even for mankind, if something would go wrong. But the question remains: How safe is “safe enough”?
The promoter, pLac_lux hybrid promoter (BBa_K091100), of the freeze subsystem is negatively regulated through LuxI and positively regulated through lactose. LuxI production is induced by L-arabinose.
PIJLTJESSCHEMA
BIOBRICKSCHEMA
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