Team:Osaka/week6

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==September 5(Mon)==
==September 5(Mon)==
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:10px;" cellpadding="5"
+
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
|+Table 7
|+Table 7
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
|-
|-
-
|1|| -||2h||0.8583||0.1567
+
|1|| -||-||0.8583||0.1567
|-
|-
|2||400μl||2h||1.1523||0.1447
|2||400μl||2h||1.1523||0.1447

Revision as of 12:41, 5 September 2011


Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

  1. Absorbance measurement
Table 7
NameIPTG(0.1M)incubationOD600A474
1 --0.85830.1567
2400μl2h1.15230.1447
3400μl1h0.55830.1126
440μl2h1.02970.1878
540μl1h0.65760.1736


September 6(Tue)

September 7(Wed)

September 8(Thu)

September 9(Fri)

September 10(Sat)

Back to Notebook

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