Team:Osaka/week6

From 2011.igem.org

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==September 5(Mon)==
==September 5(Mon)==
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
|+Table 1
|+Table 1
!ID!!Part Name!!Resistance!!Description
!ID!!Part Name!!Resistance!!Description
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|1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd)
|1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd)
-
|-
 
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|3-6H||<bbpart>BBa_K274100</bbpart>||A||CrtEBI with rbs
 
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|-
 
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|2-18H||<bbpart>BBa_K118014</bbpart>||A||rbs+crtE (geranylgeranyl pyrophosphate synthase)
 
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|-
 
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|2-16N||<bbpart>BBa_K118006</bbpart>||A||rbs+crtB (phytoene synthase)
 
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|-
 
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|2-18H||<bbpart>BBa_K118005</bbpart>||A||rbs+crtI (phytoene dehydrogenase)
 
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|-
 
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|1-17P||<bbpart>BBa_I742155</bbpart>||A||crtY (lycopene cyclase) with native rbs
 
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|-
 
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|1-1G||<bbpart>BBa_J04450</bbpart>||A||construction plasmid containing mRFP coding device
 
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|-
 
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|1-3A||<bbpart>BBa_J04450</bbpart>||C||construction plasmid containing mRFP coding device
 
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|1-5A||<bbpart>BBa_J04450</bbpart>||K||construction plasmid containing mRFP coding device
 
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Revision as of 12:28, 5 September 2011


Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

Table 1
IDPart NameResistanceDescription
1-9N<bbpart>BBa_J22106</bbpart>A rec A (SOS) Promoter
1-2M<bbpart>BBa_K118022</bbpart>ARBS
1-14K<bbpart>BBa_B0040</bbpart>Agreen fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP
1-12M<bbpart>BBa_E0240</bbpart>AGFP generator
1-1D<bbpart>BBa_R0010</bbpart>Apromoter(lacI regulatd)


September 6(Tue)

September 7(Wed)

September 8(Thu)

September 9(Fri)

September 10(Sat)

Back to Notebook

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