Team:Osaka/week6

September 4(Sun)

1. Restriction digests:3-10C,1-2M
2. Gel extraction of digests
3. Ligation
   • 03:1-9N+3-6H again
4. Transformation of ligation products
5. Photospectrometry measuament

September 5(Mon)

|+Table 1 !ID!!Part Name!!Resistance!!Description |- |1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter |- |1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS |- |1-14K||<bbpart>BBa_B0040</bbpart>||A||green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP |- |1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator |- |1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd) |- |3-6H||<bbpart>BBa_K274100</bbpart>||A||CrtEBI with rbs |- |2-18H||<bbpart>BBa_K118014</bbpart>||A||rbs+crtE (geranylgeranyl pyrophosphate synthase) |- |2-16N||<bbpart>BBa_K118006</bbpart>||A||rbs+crtB (phytoene synthase) |- |2-18H||<bbpart>BBa_K118005</bbpart>||A||rbs+crtI (phytoene dehydrogenase) |- |1-17P||<bbpart>BBa_I742155</bbpart>||A||crtY (lycopene cyclase) with native rbs |- |1-1G||<bbpart>BBa_J04450</bbpart>||A||construction plasmid containing mRFP coding device |- |1-3A||<bbpart>BBa_J04450</bbpart>||C||construction plasmid containing mRFP coding device |- |1-5A||<bbpart>BBa_J04450</bbpart>||K||construction plasmid containing mRFP coding device |}

September 6(Tue)

September 7(Wed)

September 8(Thu)

September 9(Fri)

September 10(Sat)

Back to Notebook