Team:Osaka/week6

From 2011.igem.org

(Difference between revisions)
Line 12: Line 12:
==September 5(Mon)==
==September 5(Mon)==
|+Table 1
|+Table 1
-
!name!!IPTG(0.1M)!!OD600!!incubate!!A<math>474</math>
+
!ID!!Part Name!!Resistance!!Description
|-
|-
-
|1||400μl||0.8583||-||0.1567
+
|1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter
|-
|-
|1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS
|1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS
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|1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator
|1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator
|-
|-
-
|1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd)
+
|1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd)  
-
|-
+
|}
|}

Revision as of 12:27, 5 September 2011


Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

|+Table 1 !ID!!Part Name!!Resistance!!Description |- |1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter |- |1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS |- |1-14K||<bbpart>BBa_B0040</bbpart>||A||green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP |- |1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator |- |1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd) |}


September 6(Tue)

September 7(Wed)

September 8(Thu)

September 9(Fri)

September 10(Sat)

Back to Notebook

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