Team:Cornell/Week 13
From 2011.igem.org
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-took concentrations of yesterday miniprep | -took concentrations of yesterday miniprep | ||
-all 260/280nm ratio were precisely near 1.8 indicting pure DNA | -all 260/280nm ratio were precisely near 1.8 indicting pure DNA | ||
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DNA Concentration (ng/uL) | DNA Concentration (ng/uL) | ||
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RFP 1 in PSB1C3 Backbone 344.0 | RFP 1 in PSB1C3 Backbone 344.0 | ||
RFP 2 in PSB1C3 Backbone 256.4 | RFP 2 in PSB1C3 Backbone 256.4 | ||
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VIOE 2 in PSB1C3 Backbone 552.9 | VIOE 2 in PSB1C3 Backbone 552.9 | ||
VIOE 3 in PSB1C3 Backbone 400.7 | VIOE 3 in PSB1C3 Backbone 400.7 | ||
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Revision as of 20:31, 3 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
August 28th - September 3rd
Sunday, August 28
Monday, August 29
Tuesday, August 30
Afternoon - Evening lab work done by: Claire Paduano, Youjin Cho, Maneesh Gupta, James Mathew, Charlie Chung
- Digestion Reactions
- - specifics to be posted soon
- Agarose Gel Electrophoresis
- - specifics to be posted soon
- Gel Extraction and Purification of DNA
- - specifics to be posted soon (including some issues in cutting out the insert band vs. backbone band)
- NanoDrop Spectrophometry for Purified DNA Quantification
- - specific [ ]s to be posted soon
- Ligation Reactions
- - specifics to be posted soon
Wednesday, August 31
Afternoon/Evening Lab work done by: Claire Paduano and Maneesh Gupta
- Looked at ATTO590 coated chip from Aug 27th under fluorescent microscope: channels still fluorescent
- Photo to be posted soon
- Streptavidin coating and Fluorescent Probes
- Procedure to be posted soon
- Flow experiments
- Details and photos to be posted soon
Thursday, September 1
Morning lab work done by: Claire Paduano and Nancy Li
- Coated three chips with streptavidin
- Streptavidin coating
- Protocol from Gleghorn et al: http://www.ncbi.nlm.nih.gov/pubmed/20024046
- 1) 45 min in 4% (by volume) MPTMS in ethanol
- 2) 20 min in 1mM GMBS
- 3) 45 min in 25ng/mL NeutrAvidin in PBS
Evening lab work done by: Maneesh Gupta
- Did continuous flow experiments to test resilience of biotin-avitin binding
- Used same exposure (83.33) in all images taken
Friday, September 2
Saturday, September 3
Afternoon Lab accomplished by: Jim Mathew, Charlie Chung, Nancy Li
Miniprep -took concentrations of yesterday miniprep -all 260/280nm ratio were precisely near 1.8 indicting pure DNA
DNA Concentration (ng/uL)
RFP 1 in PSB1C3 Backbone 344.0 RFP 2 in PSB1C3 Backbone 256.4 RFP 3 in PSB1C3 Backbone 230.4 VIOA 1-1 in PSB1C3 Backbone 205.9 VIOA 1-2 in PSB1C3 Backbone 494.3 VIOA 1-3 in PSB1C3 Backbone 370.8 VIOA 2-1 in PSB1C3 Backbone 892.5 VIOA 2-2 in PSB1C3 Backbone 203.8 VIOA 2-3 in PSB1C3 Backbone 206.0 VIOB 1-1 in PSB1C3 Backbone 503.9 VIOB 1-2 in PSB1C3 Backbone 186.1 VIOB 1-3 in PSB1C3 Backbone 398.8 VIOB 2-1 in PSB1C3 Backbone 868.9 VIOB 2-2 in PSB1C3 Backbone 145.6 VIOB 2-3 in PSB1C3 Backbone 174.6 VIOE 1 in PSB1C3 Backbone 266.2 VIOE 2 in PSB1C3 Backbone 552.9 VIOE 3 in PSB1C3 Backbone 400.7