"
Team:Freiburg/Notebook/3 September
From 2011.igem.org
Juliimapril (Talk | contribs) (→3A-assembly:Digestion) |
Juliimapril (Talk | contribs) (→blue light receptor) |
||
| Line 48: | Line 48: | ||
To do: Trafo | To do: Trafo | ||
| + | |||
| + | |||
| + | ===Transformation=== | ||
| + | with ♥C3 Lig. and Not C3 Lig | ||
| + | |||
| + | '''Investigator: Sophie''' | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Revision as of 17:43, 3 September 2011
Contact 
Contents |
green light receptor
Testdigestion of 2a-assembly
Investigators: Julia
Continuing from 31 of Aug. [1]
Before Miniprep of 20 different colonies.
Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.
Result:
3A-assembly:Digestion
Investigators: Sandra
Digestion of:
InsertI:
- 64
- 46
with EcorI and SpeI
InsertII:
- 67
- 50
with XbaI and PstI
Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour.
3A-assembly:Ligation
Investigators: Julia
blue light receptor
Stop of ligation
Investigators: Sandra
Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.
To do: Trafo
Transformation
with ♥C3 Lig. and Not C3 Lig
Investigator: Sophie
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Picking clones
Investigators: Sandra
Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.
