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Team:Freiburg/Notebook/3 September
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Juliimapril (Talk | contribs) (→Testdigestion of 2a-assembly) |
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with XbaI and PstI | with XbaI and PstI | ||
| - | Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one | + | Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour. |
| + | ===3A-assembly:Ligation=== | ||
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| + | '''Investigators: Julia''' | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
Revision as of 16:47, 3 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
Testdigestion of 2a-assembly
Investigators: Julia
Continuing from 31 of Aug. [1]
Before Miniprep of 20 different colonies.
Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.
Result:
3A-assembly:Digestion
Investigators: Sandra
Digestion of:
InsertI:
- 64
- 46
with EcorI and SpeI
InsertII:
- 67
- 50
with XbaI and PstI
Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour.
3A-assembly:Ligation
Investigators: Julia
blue light receptor
Stop of ligation
Investigators: Sandra
Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.
To do: Trafo
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Picking clones
Investigators: Sandra
Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.
