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Team:Paris Liliane Bettencourt/Notebook/2011/09/03/
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==Danyel== | ==Danyel== | ||
| - | + | *Miniprep of '''S92'''(Pveg_spoVG_tRNA_Ter in pHM3 in MH1 cells). | |
| - | + | *Gel extraction of S82 digested on S & P and S82 digested on E & S | |
| - | + | *Ligation of: | |
S38 in S24 (CmR) | S38 in S24 (CmR) | ||
S38 in S82 (CmR) | S38 in S82 (CmR) | ||
Revision as of 13:16, 3 September 2011
Danyel
- Miniprep of S92(Pveg_spoVG_tRNA_Ter in pHM3 in MH1 cells).
- Gel extraction of S82 digested on S & P and S82 digested on E & S
- Ligation of:
S38 in S24 (CmR) S38 in S82 (CmR) S58 in S82 (CmR) (with negative controls for S24 and S82) at ratio 6:1 and 100:1
