"

From 2011.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

__NOTOC__ {{Team:Minnesota/Top}} <br> == SiL & GFP PCT == '''Project:''' Surface Display '''Date:''' Tue, 30 Aug 2011 18:38:12 GMT '''Author:''' Viki Ding (dingx055) '''Access:''' Public '''Revision History:''' :* Mon, 26 Sep 2011 18:47:02 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)<!-- Generated by Rtf2WikiMarkup - RTF to Wiki Markup Converter 1.0, Copyright © Phyrstorm Technologies, Inc. 2011 --> <!-- Technology built on the Itenso Rtf Converter Framework --> <nowiki>New vent polymerase ordered.</nowiki> <nowiki>Objective: obtain SiL and GFP fragments</nowiki> <nowiki>Silicatein (~1 kb)</nowiki> <nowiki>- 1 uL vent pol.</nowiki> <nowiki>- 3 uL dNTP</nowiki> <nowiki>- 5 uL vent buffer</nowiki> <nowiki>- 5 uL forward primer</nowiki> <nowiki>- 5 uL reverse primer</nowiki> <nowiki>- 2 uL SiL DNA</nowiki> <nowiki>- 29 uL H2O</nowiki> <nowiki>Taq mix</nowiki> <nowiki>- 5 uL forward primer</nowiki> <nowiki>- 5 uL reverse primer</nowiki> <nowiki>- 2 uL SiL DNA</nowiki> <nowiki>- 12 uL taq green mix</nowiki> <nowiki>PCR conditions</nowiki> <nowiki>95 degrees >> 4 min</nowiki> <nowiki>95 degrees >> 30 sec</nowiki> <nowiki>63 degrees >> 30 sec</nowiki> <nowiki>72 degrees >> 1 min</nowiki> <nowiki>34 cycles</nowiki> <nowiki>72 degrees >> 10 min</nowiki> <nowiki>GFP (less than 1 kb, ~950 bp)</nowiki> <nowiki>- 1 uL vent pol.</nowiki> <nowiki>- 3 uL dNTP</nowiki> <nowiki>- 5 uL vent buffer</nowiki> <nowiki>- 5 uL forward primer</nowiki> <nowiki>- 5 uL reverse primer</nowiki> <nowiki>- 2 uL GFP DNA</nowiki> <nowiki>- 29 uL H2O</nowiki> <nowiki>Taq mix</nowiki> <nowiki>- 5 uL forward primer</nowiki> <nowiki>- 5 uL reverse primer</nowiki> <nowiki>- 2 uL GFP DNA</nowiki> <nowiki>- 12 uL taq green mix</nowiki> <nowiki>PCR conditions</nowiki> <nowiki>95 degrees >> 4 min</nowiki> <nowiki>95 degrees >> 30 sec</nowiki> <nowiki>60 degrees >> 30 sec</nowiki> <nowiki>72 degrees >> 1 min</nowiki> <nowiki>34 cycles</nowiki> <nowiki>72 degrees >> 10 min</nowiki> <nowiki>Results</nowiki> <nowiki>- Sil taq: thick bright band below 500 bp</nowiki> <nowiki>- Sil vent: thick bright band at ~ 1 kb</nowiki> <nowiki>- GFP taq: thick bright band at ~ 950 bp</nowiki> <nowiki>- GFP vent: think bright band at ~ 950 bp</nowiki> {{Team:Minnesota/Bottom}}

Return to Team:Minnesota/dingx055-08302011183812-167.