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==Sunday, July 24== No colonies grew for Imp linker+KylE Colony PCR small linker+XylE Clone LacZα and GusA into K3 with new primers ==Monday, July 25== tev protease mutagenesis clone GusA and LacZα into K3 ==Tuesday, July 26== Place linkers in front of XylE Colony PCR GusA and LacZα clones Results: looked good on gel, grown up in culture ==Wednesday, July 27== Miniprep and sequence GusA and LacZα clones Results: Failure ==Thursday, July 28== Clone GusA and LacZα into K3 Results from 7/22 assemblies: Only Pr-RBS-XylE-small linker-tev cleavage site-GFP worked ==Friday, July 29== Pr-RBS-XylE-linkers'''+'''cleavage sites+GFP Colony PCR of GusA and LacZα clones Results: failure ==Saturday, July 30== Colony PCR of 7/29 assemblies Results: failure [[Team:Penn_State/Lab| Back to Wetlab]]

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