Sensor: Week 9 July 12-15

==Tuesday, July 12==
===Clone Cro and O<sub>R</sub>, Take 2 Results===
According to the sequencing results, the Cro and O<sub>R</sub> genes were successfully cloned into the K3 vector.

===cI Repressor Test Assembly, Day 31===
The following constructs were grown up in culture in order to send to sequencing for verification:

Or + K648000

Or + K648000 + J23113 + B0030 + C0051

Or + K648000 + J23113 + B0034 + C0051

Or + K648000 + J23113 + B0031 + C0051

==Wednesday, July 13==
The sensor group ordered the following oligos:

B0030 + C0051

B0031 + C0051

B0034 + C0051

The sensor group also attempted assembling two parts, one that contained both O<sub>R</sub> and the 34mCherry reporter, and one that contained Cro and the RBS B0034.

{|border="1"
!Protocol
|colspan="2" align="center" | '''Part Involved with Protocol'''
|-
|Insert PCR
|colspan="2"|B0034
|-
|rowspan="4"|Restriction Digest
|Insert using X and P:
|34mCherry
|-
|Vector using S and P:
|O<sub>R</sub>
|-
|Insert using E and S:
|B0034
|-
|Vector using E and X:
|Cro
|-
|Ligation
|colspan="2"|Or + 34Mcherry<br />Cro + B0034
|-
|Transformation/Plating
|colspan="2"| The above ligations were transformed into<br />Escherichia coli cells and plated onto kanamycin<br />resistant plates.
|}

==Thursday, July 14==
The O<sub>R</sub>+34mCherry and Cro+B0034 constructs were grown up in culture in order to extract plasmids for sequencing tomorrow.

==Friday, July 15==
The O<sub>R</sub>+34mCherry and Cro+B0034 plasmids were sent to sequencing, and the results were positive. Thus, freezer stocks from the cells containing these plasmids were made.
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