Reporter: Week 7 June 26-July 1

==Sunday, June 26==
===Insert tev Protease into K3 Vector, Take 7 Day 1===
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The plates containing the assembly from 6/25 grew colonies that only contained RFP, none of which contained the tev protease. Thus, this assembly must be done again.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The purified tev protease PCR product from 6/4 and the K3 miniprep from 6/25 were digested with XbaI and PstI in buffer 3. The digests were ligated together, then the ligation was transformed into competent Escherichia coli cells and plated onto kanamycin resistant plates.

==Monday, June 27==
===Insert tev Protease into K3 Vector, Take 8 Day 1===
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The colonies that grew overnight all contained RFP, meaning that the insertion of tev protease into K3 from 6/26 did not work. As a result, we performed the insertion again. We started with the tev protease (K316017) plasmid from the 6/8 miniprep and the K3 vector miniprep. 

{|border="1"
!Protocol
|colspan="2" align="center" |'''Part Involved in Protocol'''
|-
|Insert PCR
|colspan="2"|K316017
|-
|rowspan="2"|Restriction Digest
|Insert using XbaI and PstI:
|K316017
|-
|Vector using XbaI and PstI:
|K3
|-
|Ligation
|colspan="2"|K316017+K3
|-
|Transformation/Plating
|colspan="2"|The ligation above was transformed into<br />Escherichia coli cells then plated on a<br />kanamycin resistant plate.
|}

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