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{{:Team:NYMU-Taipei/Templates/Header/menubar}} ---- ===<font size=5><font color=cirmson>'''INTERESTING POINTS (<font color=orange>◎</font>): </font></font><font color=gray>Hummm......you mentioned about......"Realizing the Concept of ''Avatar''?"</font>'''=== :: Magnetotactic bacteria have long been regarded as an intriguing group of species exhibiting magnetotaxis with :: their fascinating bacterial organelles, magnetosomes. Also, optogenetics, the recent revolution in :: neuroscientific methodologies, has caught our attention intensively. An eagerness out of pure curiosity to :: combine and utilize these two streams of thoughts gave way to a truly ground-breaking idea, the :: '''realization of the concept of ''Avatar'''''. ---- ===<font size=5><font color=cirmson>'''GROUNDBREAKING NEWS (<font color=orange>!</font>):</font></font> <font color=gray>So we have decided to call it an......Opto-Magnetic Revolution</font>'''=== This year, NYMU-Taipei goes beyond the current realm of mainstream synthetic biology, developing a <font color=blue>'''opto-magnetic probing platform'''</font> on ''Magnetospirillum magneticum'' AMB-1 which functionally couples to channelrhodopsins (opsins that are ultra-rapidly reactive to light beams of certain wavelengths) to enable activation of neuronal circuitry in a <font color=green>'''totally wireless'''</font>, <font color=green>'''spatially and temporarily accurate'''</font>, <font color=green>'''minimally invasive'''</font>, <font color=green>'''3-dimensional multi-site stimulation enabling'''</font> fasion. ---- ===<font color=cirmson><font size=5>'''HOW CAN THAT ''EVER'' BE POSSIBLE (<font color=orange>?</font>):</font></font> <font color=gray>Basically, we focus on achieving BRET under electromagnetic field oscillations......</font>'''=== In our project, we focus heavily on '''inducible BRET by electromagnetic field oscillations''' (BRET, Bioluminescence Resonance Energy Transfer). In this line of thought, <font color=blue>'''mms13'''</font>, a transmembrane magnetite tight-binding protein on the magnetosome membrane of ''Magnetopirillum magneticum'' AMB-1 was chosen as the linker between non-contact force and biological responses. <font color=blue>'''Comformational ''wobbling'' of mms13'''</font> induced by magnetite movement under electromagnetic field oscillation is our '''''primary hypothesis'''''. Before testing on this hypothesis directly, we have designed [https://2011.igem.org/Team:NYMU-Taipei/optomagnetic-design several constructs] to prove "''lemmas''" that are believed to be foundational to this "''theory''". Computational modeling is inevitably highly stress in our project, [https://2011.igem.org/Team:NYMU-Taipei/modelling-protein-structure-champ-design check out for more]! ---- ===<font size=5><font color=cirmson>'''BUT BEFORE DREAMS COME TRUE (<font color=orange>#</font>):</font></font> <font color=gray>Yeah, yeah, we know......We were not using ''E. coli''.</font>'''=== ''Magnetospirillum magneticum'' AMB-1 has been proven to serve as a genetically manipulatable species. And we also noticed from the history of iGem that there are always some teams attempting almost every year to deal with AMB-1, but, unfortunately, those respectful movements never got their chance to appear on the jamborees. This year, we will make a change. A brand-new '''[https://2011.igem.org/Team:NYMU-Taipei/chassis magnetic chassis]''' is about to be established, and a '''complete toolkit''' containing all elements required in genetic manipulations on AMB-1 has been submitted to the registry (see [https://2011.igem.org/Team:NYMU-Taipei/chassis Chassis] for more details). ---- ===<font size=5><font color=cirmson>'''WAIT, SO YOU GONNA PUT THEM IN... (<font color=orange>§</font>):</font></font> <font color=gray>Glial cells in brain......Yes, we will make them dormant.</font>'''=== Retrospectively, '''neuroimmune response''' appears to be the major negative issue spanning all invasive neuro-stimulatory methodologies. Although we've designed the '''''injectable''''' wireless optogenetic system aimed to avoid deleterious responses surrounding the electrodes / light cables inserted into the brain tissue, our very approach, ''utilizing bacteria'', if left unresolved, may cause far more serious aftereffects. To handle this problem, we engineer our AMB-1 with genes of team Warsaw's construct in 2010. In brief, '''intracellularity with controlled cell count''' is our goal of this part. We've designed a timeline of future applications with optomagnetic AMB-1, where <font color=blue>'''stable ''in vitro'' symbiosis within glial cells precedes injection'''</font> on the schedule. And, in our design <font color=red>'''we plan to inject glial cells containing AMB-1, not bacteria (AMB-1 in this case) into the brain'''</font>, to minimize any possible immune responses. See [https://2011.igem.org/Team:NYMU-Taipei/immunological-solution Immunological solution] for detailed explanations. ---- ===<font size=5>'''<font color=green>What's Next?</font>'''</font>=== For those who want to enjoy the ''context'' of our project (including what obstacles do we face, what candidate solutions do we adopt, defining the niche and drawbacks of our project and so on...), we strongly recommend you join us on the '''[https://2011.igem.org/Team:NYMU-Taipei/background-review Journey to ''Making A Difference'']'''. For those who are well-equipped with knowledge and power, you are sincerely invited to check upon our '''experimental designs''' ('''[https://2011.igem.org/Team:NYMU-Taipei/optomagnetic-design Optomagnetic Design]''' / '''[https://2011.igem.org/Team:NYMU-Taipei/immunological-solution Immunological Solution]''') for the panorama of our project.

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