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'''Materials:''' Bacterial sample LB broth 15 ml centrifuge tube '''Procedure:''' 1. Add 2ml of sterile LB broth into a 15ml centrifuge tube. 2. Inoculate the tube with 50ul of liquid bacterial sample or a single colony from an agar plate. 3. Incubate at 37°C from one evening to the next morning. (''"overnight"'') [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''BACK''']

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