Team:Minnesota/davis950-09092011121301-119

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== Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8 ==

'''Project:''' Regulatory

'''Date:''' Fri, 09 Sep 2011 12:13:01 GMT

'''Author:''' Nathan Davis (davis950)

'''Access:''' Public

'''Revision History:'''

:* Fri, 09 Sep 2011 12:19:11 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)
:* Fri, 09 Sep 2011 12:19:30 GMT (davis950): entry data updated by davis950 (id=2)<!-- Generated by Rtf2WikiMarkup - RTF to Wiki Markup Converter 1.0, Copyright © Phyrstorm Technologies, Inc. 2011 -->
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<nowiki>Purpose: to digest pUC-pcyA-ho1 and pUC-pTet-Cph8 with appropriate restriction enzymes to put pTet-Cph8 in pUC-pcyA-ho1.</nowiki>



<nowiki>Procedure:</nowiki>



<nowiki>The following digestion reactions were prepared:</nowiki>



* <nowiki>16 ul ddH2O</nowiki>

* <nowiki>1 ul SpeI</nowiki>

* <nowiki>1 ul PstI</nowiki>

* <nowiki>3 ul NE buffer 2</nowiki>

* <nowiki>10 ul pUC-pcyA-ho1 miniprep product (139 ng/ul)</nowiki>



* <nowiki>16 ul ddH2O</nowiki>

* <nowiki>1 ul XbaI</nowiki>

* <nowiki>1 ul PstI</nowiki>

* <nowiki>3 ul NE buffer 2</nowiki>

* <nowiki>10 ul pUC-pTet-Cph8 (105 ng/ul)</nowiki>



<nowiki>Digest reactions placed in 37 degree C incubator overnight.</nowiki>



<nowiki>Results: None</nowiki>


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